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Sodium Acetate Buffer Protocol and preparation
Sodium Acetate Buffer summary of use
Sodium Acetate Buffer Protocol and preparation mainly used for protein crystallization, staining gels, and protein electrophoresis. It is also a popular buffer in hematology. Sodium acetate has an acetate group with sodium that is bound to oxygen that is suitable buffer for ranges from pH 3.5 to 5.5.
Sodium acetate buffer is proper buffer for enzymology, nucleic acid precipitation protocols, dialysis and as an elution buffer in affinity chromatography. It is a mildly acidic buffer and stable composition that can be added to other buffers. For example, Sodium acetate buffer can be added to Citrate buffer, Ammonium acetate, Potassium acetate and Lithium chloride in different DNA and PCR protocols. Also, it can be mixed with ammonium sulfate in protein purification methods.
Sodium acetate buffer is affordable and safe for applicants and the environment. Sodium acetate buffer is stable for long term and is a resistant buffer for light, temperature change and divalent ions like Mg, Zn, Ca, and Fe. Also, it is not as sensitive as other biological buffers to some microbial contamination.
In Immunoassay Sodium acetate buffers mostly are used to dialysis of Abs and Ag to resolve the Ammonium acetate, Ethanol or heat shocked precipitated proteins. In some articles researchers have used Sodium acetate buffers for making ELISA TMB Solution.
Sodium Acetate Buffer Protocol and preparation
Sodium Acetate Buffer (PH: 5.2) preparation
Prepare 950 mL of distilled water in a suitable container.
- Add 6.256 g of Sodium Acetate to the solution.
- Add 1.425 g of Acetic Acid to the solution.
- Adjust pH to 5.2 using HCl or NaOH
- Add distilled water until the volume is 1 L.
Note1: Do not adjust PH with Acetic acid, because it can change buffer molarity.
Note2: Sodium acetate buffer PH 4.5 – 5.2 is usually a standard buffer for immunochromatography elution and protein dialyzing.